Wigan star Victor Moses is set to join Chelsea for £9m, the Daily Mirror say.It is claimed that Latics chairman Dave Whelan has agreed to a deal worth less than the £10m he insisted it would take to prise the former Crystal Palace forward away from Wigan.Moses is now tipped to discuss personal terms and complete his move to Stamford Bridge.The Mirror also say Chelsea plan make-or-break talks with Marseille on Monday to try to land Spanish right-back Cesar Azpilicueta – and that QPR’s Joey Barton is on the verge of a season-long loan move to Blackpool.It is claimed Barton is open to the idea of joining the Tangerines and that Blackpool are keen to sign him in order to get one over their neighbours Fleetwood Town.Barton, who is banned for the first 12 matches of the season, has been training with Fleetwood and it has been suggested that he could join them on loan.This page is regularly updated.See also:Saints confident of seeing off Chelsea’s interest in youngster – reportFollow West London Sport on TwitterFind us on Facebook
Chelsea boss Antonio Conte has made four changes for the game at Stamford Bridge, where Eden Hazard and Cesar Azpilicueta are substitutes.Cesc Fabregas has been recalled to the side and there are also starting places for Antonio Rudiger, Willian and Pedro.Swansea, who are unchanged, are without defender Federico Fernandez because of a family bereavement.On-loan Chelsea forward Tammy Abraham is ineligible to play against the Blues.Chelsea: Courtois; Rudiger, Christensen, Cahill; Zappacosta, Fabregas, Kante, Alonso; Willian, Morata, Pedro.Subs: Caballero, Azpilicueta, Ampadu, Moses, Drinkwater, Bakayoko, Hazard. Swansea: Fabianski, Naughton, Van der Hoorn, Mawson, Olsson, Roque Mesa, Ki, Carroll, Sanches, Ayew, Bony.Subs: Nordfeldt, Rangel, Clucas, Fer, Dyer, Routledge, McBurnie. Follow West London Sport on TwitterFind us on Facebook
The cell is quicker than the eye of our best scientific instruments. Biochemists and biophysicists are nearing closer to watching cellular magic tricks in real time but aren’t quite there yet. They know it’s just a trick of the eye, but it sure is baffling how cellular machines pull off their most amazing feats. Think, but don’t blink:Knot Wizardry: Proteins needing a fold go into a private dressing room (05/05/2003). The most glamorous and well-equipped room, the GroEL-GroES chaperone, helps the star emerge just right. How it does this is as puzzling as watching a magician untie a Gordian knot under a kerchief. There are thousands of wrong ways a protein could fold; how does the chaperone always perform the trick correctly? Some of the bonds between domains (disulfide bridges) are a long way apart. What brings them together, and what keeps the wrong bridges from forming? Some scientists at Howard Hughes Medical Institute, writing in PNAS,1 cheated and built the chaperone with one door open so they could peek inside. They still couldn’t figure it out completely. Something in the chaperone creates conditions that favor the correct “native” fold, but also fix the mistakes before the prima donna protein emerges. Somehow they do this without any ATP energy cost. “We conclude that folding in the GroEL-GroES cavity can favor the formation of a native-like topology, here involving the proper apposition of the two domains of TG [trypsinogen, the enzyme in the experiment]; but it also involves an ATP-independent conformational ‘editing’ of locally incorrect structures produced during the dwell time in the cis cavity.”Speed Solve: Maybe you’ve watched a blindfolded man solve a Rubik’s cube in seconds and wondered how it was done. You can imagine the bewilderment of German and Swiss scientists watching a protein fold in far less time. Protein chains of hundreds of amino acids have to explore a vast space of possible folds yet arrive at the one correct fold, often in fractions of a second. These scientists, writing in PNAS,2 used lasers to try to figure out in slo-mo how this happens. As with a Rubik’s cube, there are billions of ways a protein could fold incorrectly. Parts of a nascent protein chain form loops in the process of solving the puzzle. “Exponential kinetics observed on the 10 to 100-ns time scale [ns=nanosecond, a billionth of a second] are caused by diffusional processes involving large-scale motions that allow the polypeptide chain to explore the complete conformational space,” they said. “The presence of local energy minima [e.g., loops] reduces the conformational space and accelerates the conformational search for energetically favorable local intrachain contacts.” To catch these loops, they had to look fast. “Complex kinetics of loop formation were observed on the 50- to 500-ps [picosecond] time scale,” they noted. A picosecond is a trillionth of a second. Good thing they had lasers that could flash up to a femtosecond (quadrillionth of a second), or it would all be a blur.Levitation: With a feat better than defying gravity, “Cytochrome c oxidase catalyzes most of the biological oxygen consumption on Earth, a process responsible for energy supply in aerobic organisms,” wrote a Finnish team also publishing in PNAS.3 To do this trick, the enzyme has to go against the force. Scientists like to talk in dispassionate language, but they called this enzyme “remarkable,” so they must have liked the magic act. “This remarkable membrane-bound enzyme also converts free energy from O2 reduction to an electrochemical proton gradient by functioning as a redox-linked proton pump,” they remarked about the remarkable. The way this pump works has “remained elusive,” even though most of the structure has been known. With special spectroscopic and electrometric techniques, they were able to observe the trick in real time. Abracadabra led to eureka: “The observed kinetics establish the long-sought reaction sequence of the proton pump mechanism and describe some of its thermodynamic properties.” OK, tell us. What’s the secret?The 10-microsecond electron transfer to heme [iron complex] a raises the pKa of a “pump site,” which is loaded by a proton from the inside of the membrane in 150 microseconds. This loading increases the redox potentials of both hemes a and a3, which allows electron equilibration between them at the same rate. Then, in 0.8 ms, another proton is transferred from the inside to the heme a3/CuB center, and the electron is transferred to CuB. Finally, in 2.6 ms, the preloaded proton is released from the pump site to the opposite side of the membrane.So, there. Now you know the trick. Uh, how’s that again? Actually, they only figured out part of the trick; “some important details remain unsolved,” they confessed, “e.g., the identity of the proton-accepting pump site above the hemes.” Their diagram of the enzyme looks for all the world like magician’s tightly-cupped hands, with the active site secreted within. Maybe this could be dubbed sleight-of-enzyme.In the introduction to this last paper, the authors described how the enzyme is essential to all life. It is a key player in the transfer of electrons and protons that feed the ATP synthase motors that produce ATP – the universal energy currency for all living things. Water is produced in the process that generates oxygen (in plants) and consumes it (in animals). These reactions would not occur without the machinery to drive them against the physical forces of diffusion. The scientists are converging on a mechanical description of how the pumping action works. “Each of the four electron transfer steps in the catalytic cycle of CcO [cytochrome c oxidase] constitutes one cycle of the proton pump, which is likely to occur by essentially the same mechanism each time,“ they said. “Here, we report on the internal electron transfer and charge translocation kinetics of one such cycle, which is set forth by fast photoinjection of a single electron into the oxidized enzyme.”1Eun Sun Park, Wayne A. Fenton, and Arthur L. Horwich, “Disulfide formation as a probe of folding in GroEL-GroES reveals correct formation of long-range bonds and editing of incorrect short-range ones,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0610989104, published online before print February 5, 2007.2Fierz, Satzger et al, “Loop formation in unfolded polypeptide chains on the picoseconds to microseconds time scale,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0611087104, published online before print February 6, 2007.3Belevich et al, “Exploring the proton pump mechanism of cytochrome c oxidase in real time,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0608794104, published online before print February 9, 2007.We may not be able to tell how it’s done, but we all know that a stage magic trick is just an illusion. But a good trick doesn’t just happen, either. It takes a lot of intelligent design to put on a good show. Split-second timing, carefully engineered props, trained assistants, planning, and precise manipulation are all required. If and when we figure out all the cell’s tricks, it should produce even more awe than a childish belief in magic. It should produce a deeper respect for the planning and execution of a well-designed show – and a hearty round of applause. Need we say how disappointing it was for Nature to submit this Stupid Evolution Quote of the Week about the same time as this last paper appeared: “The invention of oxygenic photosynthesis was a small step for a bacterium, but a giant leap for biology and geochemistry. So when and how did cells first learn to split water to make oxygen gas?” (John F. Allen and William Martin, “Evolutionary biology: Out of thin air,” Nature 445, 610-612, 8 February 2007). Shamelessly, they continued on and on: “Biologists agree that cyanobacteria invented the art of making oxygen, but when and how this came about remain uncertain.” It appears that some childish scientists still believe in magic. We hope the growing brightness of design emerging from cell biology will not cause too much pain as it shatters their illusions. If they maintain their illusions in spite of the evidence, though – well, willful blindness is its own punishment.(Visited 14 times, 1 visits today)FacebookTwitterPinterestSave分享0
The Odisha government presented a ₹1,39,000 crore budget for 2019-20 in the State Assembly on Friday with focus on agriculture, poverty reduction, rural development and empowerment of women and youth.Presenting the budget, State Finance Minister Niranjan Pujari said the outlay is proposed to be financed through revenue receipts of ₹1,15,266 crore and borrowing and other receipts of ₹23,734 crore.Mr. Pujari presented the budget in two parts – first as agriculture budget and the second as general budget. The proposed agriculture budget, with an outlay of ₹20,714 crore, is about three times the size of the first agriculture budget presented in 2013-14.A sum of ₹5,611 crore has been proposed for the KALIA (Krushak Assistance for Livelihood and Income Augmentation) scheme to provide financial support to about 75 lakh farmer families.‘Lifeline of economy’“Agriculture remains the lifeline of our economy, contributing to our food security and providing livelihood to the vast majority of our people,” said Mr. Pujari while presenting the agriculture budget.More than 400 suggestions were received from people and these were carefully considered while preparing the budget, Mr. Pujari saidStating that the budget focuses on boosting agriculture production and productivity in other sectors, Mr. Pujari said restoration work in the districts affected by Cyclone Fani and creation of disaster-resilient physical assets would be the government’s priority.“With substantial increase in investment in infrastructure we intend to increase economic activities and attract manufacturing and service industries especially in the MSME sector,” he said.Observing that education and skills were the key drivers of human development and enablers of employment and livelihood, the Finance Minister proposed an allocation of ₹19,492 crore for the sector.Mr. Pujari also announced that the total budgetary allocation for the State’s Health and Family Welfare Department has been increased by 10% to ₹6,828 crore in 2019-20.Paperless budgetingAs a green initiative, the government this year reduced physical printing of budget documents from 500 sets to 200 sets and would distribute the budget documents in electronic form to stakeholders.“Overall, it’s a pro-farmer, pro-women, pro-poor and growth-oriented budget,” said Chief Minister Naveen Patnaik reacting to the budget.
Newcastle striker Andy Carroll set for Brighton chanceby Paul Vegasa month agoSend to a friendShare the loveNewcastle United striker Andy Carroll is set to be named on the bench against Brighton this weekend.It will be Carroll’s second debut for Newcastle after joining as a free agent in the summer.Carroll visited a specialist in London last week and was given the okay to speed up his recovery, according to the Daily Mail.The former Liverpool man has trained well over the past two days, giving Steve Bruce enough confidence to include him in the match-day squad to face the Seagulls. About the authorPaul VegasShare the loveHave your say
TOKYO — The Latest on the arrest and investigation into alleged financial misconduct by Nissan’s chairman Carlos Ghosn (all times local):6:15 p.m.France’s finance minister wants carmaker Renault to replace its once-superstar CEO Carlos Ghosn, who is facing accusations he under-reported income at partner company Nissan.Renault will hold a board meeting Tuesday to discuss next steps after Ghosn’s arrest in Japan and planned dismissal from Nissan.Ghosn runs Renault, Nissan and the Renault-Nissan-Mitsubishi alliance that he helped turn into the world’s biggest car-seller last year.French Finance Minister Bruno Le Maire told broadcaster France-Info on Tuesday that Ghosn is not in a position to lead the Renault Group because of the accusations. He urged the board to name temporary leadership instead.Le Maire said French authorities have examined Ghosn’s tax situation in France but have found no wrongdoing.Renault officials refused further comment. Shares in Renault continued to fall Tuesday.___1:30 p.m.The arrest of Nissan Motor Co.’s chairman Carlos Ghosn on charges he underreported his income and misused company funds caused the company’s shares to tumble and shocked many in Japan who view him as something of a hero.Prosecutors said they were holding Ghosn and another Nissan executive for allegedly collaborating to falsify securities statements and underreport Ghosn’s income for five years.Chief Cabinet Secretary Yoshihide Suga said Tuesday that the government was watching for economic fallout from the scandal at one of the world’s biggest automakers.Ghosn was arrested Monday and Nissan’s CEO said the company’s board will meet on Thursday to approve his dismissal. Prosecutors refused to say where he was being held in Tokyo.The Brazilian-born executive was credited with engineering Nissan’s revival over two decades.The Associated Press
WASHINGTON — Three days before a U.S.-China summit, the top U.S. trade official is blasting Beijing for imposing “egregious” taxes on American-made cars.In a statement Wednesday, U.S. Trade Rep. Robert Lighthizer complained that China slaps 40 per cent tariffs on U.S. auto imports — more than the 15 per cent tariffs it imposes on other countries and the 27.5 per cent U.S. tax on Chinese auto imports.Lighthizer said the president had directed him to “examine all available tools to equalize the tariffs applied to automobiles.”The statement comes before a dinner meeting Saturday in Buenos Aires, Argentina, between President Donald Trump and his Chinese counterpart, Xi Jinping. The two leaders are expected to seek a resolution to a trade dispute between their countries that has shaken financial markets and threatened the global economy.Paul Wiseman, The Associated Press
Canbriam says that its Altares asset continues to deliver consistent performance with production during the second quarter averaging approximately 40,400 barrels of oil equivalent per day, 16 percent of which was liquids.The company reaffirmed 2018 production expectations of between 37,000 and 39,000 boe/d, which includes routine downtime related to summer drilling and completion activities. CALGARY, A.B. – Canbriam Energy announced on Tuesday that it has sold some non-core Montney lands in Northeast B.C. for $50 million.The company said that the assets had no associated production, and the disposition closed on July 30, 2018.“The disposition of non-core acreage supports the core development at our Altares Montney assets and the ongoing deleveraging of our balance sheet,” said Paul Myers, Canbriam’s President & Chief Executive Officer.
He added that at this point it’s not known when the trail will be reopening, but that the City will be notifying residents via its Facebook page. FORT ST. JOHN, B.C. – City officials say that one of the walking trails located near the Fort St. John Hospital will be closed until further notice.Communications Coordinator Ryan Harvey said that work crews were attempting to resolve drainage issues near the walking trail along the south side of the hospital property when some of the equipment caused damage to the trail itself.Harvey said that the City is currently working with engineers to try to get the drainage issues in the area resolved while also determining what repairs need to be performed on the trail itself.