first_imgThe young woman killed when her car collided with a bus in Co Donegal was the sixth person to be killed on the same stretch of road in recent years.The dead woman, named locally as Siobhan Hutcheon from Ardmore Manor in Muff, was killed instantly when her car was in collision with a Bus Eireann coach yesterday afternoon.The accident happened just after 3.30pm on Saturday on the N13 between Letterkenny and Newtowncunningham close to the townland of Ardee. Ms Hutcheon, who was in her 30s, was pronounced dead at the scene.Three children in the car were rushed to Altnagelvin Hopsital in Derry and two are understood to be in a stable but critical condition.Accident scene investigators spent a number of hours at the scene of the crash on Saturday afternoon.The dead woman’s body was taken to Letterkenny General Hospital where a post mortem was due to take place. It has now been revealed that six people including Ms Hutcheon have been killed in the past five years on the same stretch of road.Bizarrely two young men were killed on the exact same date as the latest fatal accident five years agoLife-long pals Gary McLaughlin, 21, and Darren Downey, 19, were killed in a single vehicle accident on May 4th, 2009.All victims including Ms Hutcheon wer rpayed for at the local All Saints Church in Manorcunningham today.Last night local county councillor Paul Canning, who lives close to the scene of the crash, revealed there was a plan to look at straightening the dangerous stretch of road. The road comes into a series of tight bends from two straight and long stretches from both the Letterkenny and Derry directions.Cllr Canning revealed “It is perhaps something we should look at again. I recall we even went as far as to consider purchasing land in the area in the event of straightening that stretch of road.“Perhaps we should at that again in the light of yet another crash and another lost life,” he said.DEAD WOMAN WAS SIXTH PERSON KILLED ON SAME STRETCH OF ROAD IN RECENT YEARS was last modified: May 4th, 2014 by StephenShare this:Click to share on Facebook (Opens in new window)Click to share on Twitter (Opens in new window)Click to share on LinkedIn (Opens in new window)Click to share on Reddit (Opens in new window)Click to share on Pocket (Opens in new window)Click to share on Telegram (Opens in new window)Click to share on WhatsApp (Opens in new window)Click to share on Skype (Opens in new window)Click to print (Opens in new window)Tags:crashdonegalGardaiNewtowncunninghamSiobhan Hutcheonlast_img read more

Victor Moses ‘will complete £9m move to Chelsea’

first_imgWigan star Victor Moses is set to join Chelsea for £9m, the Daily Mirror say.It is claimed that Latics chairman Dave Whelan has agreed to a deal worth less than the £10m he insisted it would take to prise the former Crystal Palace forward away from Wigan.Moses is now tipped to discuss personal terms and complete his move to Stamford Bridge.The Mirror also say Chelsea plan make-or-break talks with Marseille on Monday to try to land Spanish right-back Cesar Azpilicueta – and that QPR’s Joey Barton is on the verge of a season-long loan move to Blackpool.It is claimed Barton is open to the idea of joining the Tangerines and that Blackpool are keen to sign him in order to get one over their neighbours Fleetwood Town.Barton, who is banned for the first 12 matches of the season, has been training with Fleetwood and it has been suggested that he could join them on loan.This page is regularly updated.See also:Saints confident of seeing off Chelsea’s interest in youngster – reportFollow West London Sport on TwitterFind us on Facebooklast_img read more

Cells Perform Nanomagic

first_imgThe cell is quicker than the eye of our best scientific instruments.  Biochemists and biophysicists are nearing closer to watching cellular magic tricks in real time but aren’t quite there yet.  They know it’s just a trick of the eye, but it sure is baffling how cellular machines pull off their most amazing feats.  Think, but don’t blink:Knot Wizardry:  Proteins needing a fold go into a private dressing room (05/05/2003).  The most glamorous and well-equipped room, the GroEL-GroES chaperone, helps the star emerge just right.  How it does this is as puzzling as watching a magician untie a Gordian knot under a kerchief.  There are thousands of wrong ways a protein could fold; how does the chaperone always perform the trick correctly?  Some of the bonds between domains (disulfide bridges) are a long way apart.  What brings them together, and what keeps the wrong bridges from forming?    Some scientists at Howard Hughes Medical Institute, writing in PNAS,1 cheated and built the chaperone with one door open so they could peek inside.  They still couldn’t figure it out completely.  Something in the chaperone creates conditions that favor the correct “native” fold, but also fix the mistakes before the prima donna protein emerges.  Somehow they do this without any ATP energy cost.  “We conclude that folding in the GroEL-GroES cavity can favor the formation of a native-like topology, here involving the proper apposition of the two domains of TG [trypsinogen, the enzyme in the experiment]; but it also involves an ATP-independent conformational ‘editing’ of locally incorrect structures produced during the dwell time in the cis cavity.”Speed Solve:  Maybe you’ve watched a blindfolded man solve a Rubik’s cube in seconds and wondered how it was done.  You can imagine the bewilderment of German and Swiss scientists watching a protein fold in far less time.  Protein chains of hundreds of amino acids have to explore a vast space of possible folds yet arrive at the one correct fold, often in fractions of a second.  These scientists, writing in PNAS,2 used lasers to try to figure out in slo-mo how this happens.    As with a Rubik’s cube, there are billions of ways a protein could fold incorrectly.  Parts of a nascent protein chain form loops in the process of solving the puzzle.  “Exponential kinetics observed on the 10 to 100-ns time scale [ns=nanosecond, a billionth of a second] are caused by diffusional processes involving large-scale motions that allow the polypeptide chain to explore the complete conformational space,” they said.  “The presence of local energy minima [e.g., loops] reduces the conformational space and accelerates the conformational search for energetically favorable local intrachain contacts.”  To catch these loops, they had to look fast.  “Complex kinetics of loop formation were observed on the 50- to 500-ps [picosecond] time scale,” they noted.  A picosecond is a trillionth of a second.  Good thing they had lasers that could flash up to a femtosecond (quadrillionth of a second), or it would all be a blur.Levitation:  With a feat better than defying gravity, “Cytochrome c oxidase catalyzes most of the biological oxygen consumption on Earth, a process responsible for energy supply in aerobic organisms,” wrote a Finnish team also publishing in PNAS.3  To do this trick, the enzyme has to go against the force.    Scientists like to talk in dispassionate language, but they called this enzyme “remarkable,” so they must have liked the magic act.  “This remarkable membrane-bound enzyme also converts free energy from O2 reduction to an electrochemical proton gradient by functioning as a redox-linked proton pump,” they remarked about the remarkable.  The way this pump works has “remained elusive,” even though most of the structure has been known.  With special spectroscopic and electrometric techniques, they were able to observe the trick in real time.  Abracadabra led to eureka: “The observed kinetics establish the long-sought reaction sequence of the proton pump mechanism and describe some of its thermodynamic properties.”  OK, tell us.  What’s the secret?The 10-microsecond electron transfer to heme [iron complex] a raises the pKa of a “pump site,” which is loaded by a proton from the inside of the membrane in 150 microseconds.  This loading increases the redox potentials of both hemes a and a3, which allows electron equilibration between them at the same rate.  Then, in 0.8 ms, another proton is transferred from the inside to the heme a3/CuB center, and the electron is transferred to CuB.  Finally, in 2.6 ms, the preloaded proton is released from the pump site to the opposite side of the membrane.So, there.  Now you know the trick.  Uh, how’s that again?  Actually, they only figured out part of the trick; “some important details remain unsolved,” they confessed, “e.g., the identity of the proton-accepting pump site above the hemes.”  Their diagram of the enzyme looks for all the world like magician’s tightly-cupped hands, with the active site secreted within.  Maybe this could be dubbed sleight-of-enzyme.In the introduction to this last paper, the authors described how the enzyme is essential to all life.  It is a key player in the transfer of electrons and protons that feed the ATP synthase motors that produce ATP – the universal energy currency for all living things.  Water is produced in the process that generates oxygen (in plants) and consumes it (in animals).  These reactions would not occur without the machinery to drive them against the physical forces of diffusion.    The scientists are converging on a mechanical description of how the pumping action works.  “Each of the four electron transfer steps in the catalytic cycle of CcO [cytochrome c oxidase] constitutes one cycle of the proton pump, which is likely to occur by essentially the same mechanism each time,“ they said.  “Here, we report on the internal electron transfer and charge translocation kinetics of one such cycle, which is set forth by fast photoinjection of a single electron into the oxidized enzyme.”1Eun Sun Park, Wayne A. Fenton, and Arthur L. Horwich, “Disulfide formation as a probe of folding in GroEL-GroES reveals correct formation of long-range bonds and editing of incorrect short-range ones,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0610989104, published online before print February 5, 2007.2Fierz, Satzger et al, “Loop formation in unfolded polypeptide chains on the picoseconds to microseconds time scale,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0611087104, published online before print February 6, 2007.3Belevich et al, “Exploring the proton pump mechanism of cytochrome c oxidase in real time,” Proceedings of the National Academy of Sciences USA, 10.1073/pnas.0608794104, published online before print February 9, 2007.We may not be able to tell how it’s done, but we all know that a stage magic trick is just an illusion.  But a good trick doesn’t just happen, either.  It takes a lot of intelligent design to put on a good show.  Split-second timing, carefully engineered props, trained assistants, planning, and precise manipulation are all required.  If and when we figure out all the cell’s tricks, it should produce even more awe than a childish belief in magic.  It should produce a deeper respect for the planning and execution of a well-designed show – and a hearty round of applause.    Need we say how disappointing it was for Nature to submit this Stupid Evolution Quote of the Week about the same time as this last paper appeared: “The invention of oxygenic photosynthesis was a small step for a bacterium, but a giant leap for biology and geochemistry.  So when and how did cells first learn to split water to make oxygen gas?” (John F. Allen and William Martin, “Evolutionary biology: Out of thin air,”  Nature 445, 610-612, 8 February 2007).  Shamelessly, they continued on and on: “Biologists agree that cyanobacteria invented the art of making oxygen, but when and how this came about remain uncertain.”    It appears that some childish scientists still believe in magic.  We hope the growing brightness of design emerging from cell biology will not cause too much pain as it shatters their illusions.  If they maintain their illusions in spite of the evidence, though – well, willful blindness is its own punishment.(Visited 14 times, 1 visits today)FacebookTwitterPinterestSave分享0last_img read more

Malloom® 12PC Baby Girls Elastic Bowknot Flower Hairband Photography Headbands : Nice variety colours-all the exact same design headbands

first_imgI appreciate the wide range of colors. They match all my daughter’s outfits. They’re a good measurement and rather good high-quality for the selling price. My daughter is four months previous and really large for her dimensions, and i feel she could get a couple of months dress in out of them. I imagine with a smaller sized baby they will final for a longer time.Properly madevibrant coloursyou obtain you finish up with a person to match all outfits your baby hasthey suit my 9 thirty day period outdated baby with rook to hrow but they do not slide off little bit they also in shape my 4 year old also apart from the pink 1 which is about a centimetre scaled-down but as they are handmade youd hope that and given that they are intended for toddlers i dont see that as a flaw. 1 of them had a couple unfastened threads 9n vut that was uncomplicated rectified. Total exyremely content with my buy and i intend to obtain the other pack they do.Malloom® 12PC Baby Girls Elastic Bowknot Flower Hairband Photography HeadbandsHeadband length:34-46cmMaterial: ClothQuantity: 12Very cute personalityThe hair band is pure hand-made: the mothers to the baby with the belt before you clean out excess hair and short hair, give your baby with, in order to avoid straying into the mouth of the baby, please know (a little hair loss is normal now,! wigs are made of plastic adhesive tape, sent to bring more or less residual glue will not affect the appearance equipped with, please note if you mind)Practically 1 for every single outfit for my daughter she appears to be gorgeous in them.These headbands r outstanding my daughter seems to be excellent in them. These headbands r brilliant my daughter looks excellent in them. The only issue was 1 of them the cloth was not overlapped adequately on the beneath of a single of them and it left a mark on her head.These headbands are superb. They in good shape my nine week outdated baby girl’s head seriously nicely. They will not depart any marks or dents and are a great sizing with area to grow. The colors are wonderful and the headbands are a discount.Malloom® 12PC Baby Girls Elastic Bowknot Flower Hairband Photography Headbands :last_img read more

Diversity is the key to cover crops

first_imgShare Facebook Twitter Google + LinkedIn Pinterest The most common use of cover crops among farmers is simply planting cereal rye after harvesting a cash crop in the fall. It is easy to plant just the cereal rye and be done, but cover crops are best used with a goal in mind.“You need to ask why you are planting it and what you want to accomplish from it,” said Rob Albers, the National Sales Manager at Center Seeds. “Diversity is a major component in being successful with cover crops. It adds more benefits than a simple monoculture stand.”An easy way to obtain a diversified cover crop is to use a mix of legumes, grasses, and brassicas. The legumes will produce organic nitrogen while the grasses and brassica scavenge nutrients and some brassicas are good for breaking up compaction. That combination will give a good stand, but an even better stand will have diversity within each of those three categories as well — several legumes, a few grasses, and a couple brassicas all in the same mix.To many, this type of mix can seem a little overwhelming. However, it is simple to accomplish and does not have to cost a fortune.“It’s the presence of the multispecies that’s important, not the poundage. Instead of drilling 60 pounds of straight cereal rye, you can break that up by putting in smaller amounts of cereal rye, oats, barley, annual ryegrass, winter peas, crimson clover, vetch, cowpeas, radish, and/or rapeseed,” Albers said.Diversification is proving to be increasingly important as the different root systems provide nutrients to the soil biology as well as absorbing different nutrients from the soil. Some roots will reach deeper into the soil profile whereas others will be more dense and fibrous, gathering nutrients from other parts of the soil. This is when the hyphae start interconnecting, which are important to extract nutrients to support growth in poor soils.Albers described this process as a spider spinning a web — the bigger their web, the more opportunity they have for food. That’s exactly what is happening under the soil surface when multiple plant species are grown together; the hyphae can extend for miles when they are receiving the proper nutrients from the soil and not disturbed by tillage.In order to figure out which combination would provide soils with the most benefit, the following questions need to be asked:• What will be planted next year? Some crops are more beneficial before a soybean crop and others before a corn crop.• Are there any herbicide issues or residuals? The cover crops to use may be limited or not even an option with the presence of residual herbicides.• In what time frame will the cover crops be planted? This will help decide if a warm or cool season crop should be planted and what crops will have the most benefit.Based on these simple questions, there is no general cover crop recommendation for every farm or every field. In a wet year like this, many people have started planting cover crops in July. Some warm season options for this early planting would be sorghum-sudangrass, pearl millet, Berseem clover, or sunn hemp as well as blends that include sorghum-sudan, radish, and sunn hemp or a blend with sorghum-sudan, sunn hemp, and buckwheat. Each of these can be seeded at a rate of about 15 to 20 pounds per acre, or even a little less, with a cost anywhere between $10 and $40 an acre depending on the seed and mixture.For later plantings in August or September, a few options would be annual ryegrass, cereal rye, spring oats, medium red clover, vetch, winter pea, radish, or rapeseed or a type of blend that includes some of those crops. The oats and rye will need to be planted a higher rate of 60 or more pounds per acre if they are not being mixed with another crop, and winter peas should be planted at a rate of 30 to 40 pounds per acre while the vetch can be planted at a rate of 10 to 20 pounds per acre. The other plants can be seeded at a rate lower than 10 pounds per acre. When these seeds are blended together, they can normally be planted at a lower rate of 15 to 20 pounds per acre and cost an average $10 to $45 an acre. Again, this is all depending on which seeds or mixtures are used.“To maximize the benefits, you have to know what you hope to accomplish with cover crops before planting them,” Albers said. “By adding diversity, you are simply increasing your odds of achieving those goals and seeing better results in your fields with cover crops.”last_img read more

Hacker Poll: Where Do You Usually Code?

first_imgGrowing Phone Scams: 5 Tips To Avoid Related Posts Why You Love Online Quizzes How to Write a Welcome Email to New Employees? klint finleycenter_img Tags:#hack#Polls 7 Types of Video that will Make a Massive Impac… Do you code at home, away from co-worker’s interruptions? Or do you prefer a public setting, perhaps with colleagues? Or do you do you work in a conventional setting, at your place of employment?Let us know where you work.Photo by Shereen Mlast_img

SQL Injection Hacker Attacks Are On The Rise. Here’s How To Defend Your Servers

first_imgdan rowinski 8 Best WordPress Hosting Solutions on the Market Last week, a hacker group claimed that it breached computer systems at 100 major universities. Team GhostShell gained access to servers at Stanford, Harvard, and the University of Michigan, among others. The technique used, SQL injection, is not new or complex, but reportedly it’s becoming increasingly common. Here’s a quick guide to defending your servers.Basic BasicsWe asked researchers at security firm Sophos to explain what an SQL injection is and how it can be stopped. Before launching into that, though, for laymen, here are a couple things you need to know about an SQL injection before learning how to stop one.SQL stands for Structured Query Language. It is an international standard for interacting with databases. Statements in SQL can retrieve, insert, create and otherwise change data in a database. Code injection is a technique used by hackers to exploit vulnerabilities in a website. “SQL injection is an old, well established method of attacking systems,” said Sophos threat researcher Fraser Howard. “It consists of inserting malicious SQL statements into an application to cause it to perform some undesirable function.”Mechanics Of An AttackUndesirable action sounds nasty. What does it mean exactly? Here are a few examples:Dump table (i.e., return a dump of the entire contents of a database table). This is a great way to steal data. Could be used to gain access to a system (dump admin password, then access the system etc.)Drop table (delete table contents). Destructive. Attackers do not necessarily gain access to the data, but they can break the system. Data may be irretrievably lost.Modify table. Insert additional data into the database table.Basically, once a SQL injection has its hooks in your database, it can do whatever the heck the malicious hacker behind it wants. Steal your data (most commonly), delete your data, change your data. “Imagine a website where page contents are stored in a database,” Howard wrote. “When you browse the site, the database is queried, and the page shows you whatever information is relevant. For example, a shopping site. You search for carrots, it queries the database and gets the price. The page you view displays this price.” A malicious hacker using SQL injection could download the store’s entire stock list, wipe it out, and/or change all the prices (or any other category of information).One further problem with SQL injection not related to theft: Hackers can change the query instructions for a Web application. So instead of the application querying its own server and obtaining information, the query can be sent to a server of the hacker’s choice. This can lead to malware infecting a user’s computer. Scary stuff, huh?How To Defend Your ServersAccording to Howard, defense against this type of attack is all about the Web application that is the door to the server. Protect that application and you protect the server. In theory, at least. Most organizations likely will remain vulnerable to a dedicated, sophisticated hacker no matter what they do. Not all hackers are so single-minded, so it makes sense to be prepared. Here are the steps Howard recommends to defend against SQL injection attacks:Secure programming. Design applications securely from the start. SQL injection is not new, and there are many books and online resources to help developers build applications that are secure against this attack. The most common vulnerability is an application that doesn’t sanity-check user input such as data entered into Web forms. If the input is not checked, an attacker can use such forms to inject malicious instructions.Firewalling. This does not replace secure programming. However, it can add a layer of defense in front of your Web server. Web application firewalls can help to block most attacks.Many organizations are vulnerable to SQL injections because they outsource their Web application development, rush production, test poorly and take little regard for security. “Recipe for disaster,” Howard said. “Lots of easy targets out there.”In security, the guidelines are usually pretty simple: Take your time, factor security into everything you do, and use common sense. Security might seem like the boring part of what you do, but if you do not pay attention to it, there is a hacker just waiting to break into your databases and steal, destroy, or alter your data. Why Tech Companies Need Simpler Terms of Servic… A Web Developer’s New Best Friend is the AI Wai…center_img Related Posts Top Reasons to Go With Managed WordPress Hosting Tags:#biz#security last_img read more